Gloveless Anaerobic Chambers offer the operator a convenient glove-free ability to handle and inspect samples under low oxygen (0-5 ppm) conditions. A special cuff and sleeve-vacuum system allows the operator to work barehanded (or with surgical gloves) inside the glove box. After the operator inserts their arms into the gloveless sleeves, a foot operated sleeve-vacuum system creates an inert atmosphere in the sleeves prior to removing the Arm Port Plugs. This permits the operator's arms to enter the glove box without compromising the anaerobic chamber atmosphere. Tactile efficiency is improved. The Arm Port Plugs are used to seal the glove box while it is idle.